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Product Details
Synonym
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
Mouse PD-L1, His Tag (PD1-M5220) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693).
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse PD-L1, His Tag (Cat. No. PD1-M5220) is more than 95% and the molecular weight of this protein is around 34-46 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse PD-L1, His Tag (Cat. No. PD1-M5220) at 10 μg/mL (100 μL/well) can bind Rabbit PD-1, Fc Tag (Cat. No. PD1-RB5251) with a linear range of 3-50 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Captured Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on Protein A Chip can bind Mouse PD-L1, His Tag (Cat. No. PD1-M5220) with an affinity constant of 2.16 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Captured Mouse PD-L1, His Tag (Cat. No. PD1-M5220) on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) with an affinity constant of 1.37 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Mouse PD-1, Fc,Avitag (Cat. No. PD1-M82F4) on SA Biosensor, can bind Mouse PD-L1 / B7-H1 Protein, His Tag (Cat. No. PD1-M5220) with an affinity constant of 3.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Mouse PD-L1 / B7-H1 Protein, His Tag (Cat. No. PD1-M5220) on HIS1K Biosensor, can bind Mouse PD-1 Protein, Mouse IgG2a Fc Tag (Cat. No. PD1-M5257) with an affinity constant of 0.227 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed death-ligand 1
- Category：
- Approved Drugs：
12 Details
- Drugs in Clinical Trials：
176 Details
- Highest Development Stage：
Approved
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