Monoclonal Anti-Nipah Post Fusion glycoprotein Antibody, Human IgG1 (2B2) (MALS verified)

Customer Reviews
Cat. No. / Size
Price
Qty
NIN-M759-100ug
€360.00
NIN-M759-1mg
€2400.00
ETA of in-stock products:3 business days

Product Details

  • Source

    Monoclonal Anti-Nipah Post Fusion glycoprotein Antibody, Human IgG1 (2B2) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    2B2

  • Species

    Mouse

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Specificity

    This product is a specific antibody specifically reacts with Nipah Post Fusion glycoprotein.

  • Application

    Application
    Recommended Usage
    ELISA
    0.03-31 ng/mL
  • Purity

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • SDS-PAGE

    Nipah Post Fusion glycoprotein SDS-PAGE

    Monoclonal Anti-Nipah Post Fusion glycoprotein Antibody, Human IgG1 (2B2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Nipah Post Fusion glycoprotein SEC-MALS

    The purity of Monoclonal Anti-Nipah Post Fusion glycoprotein Antibody, Human IgG1 (2B2) (Cat. No. NIN-M759) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Nipah Post Fusion glycoprotein ELISA

    Immobilized Nipah virus Post-Fusion glycoprotein, His Tag (Cat. No. PON-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Nipah Post Fusion glycoprotein Antibody, Human IgG1 (2B2) (Cat. No. NIN-M759) with a linear range of 0.03-1 ng/mL (QC tested).

    Protocol

Customer Reviews
Writing Reviews

Background

Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses discovered in the mid-to late 1990s that possess a broad host tropism and are known to cause severe and often fatal disease in both humans and animals. HeV and NiV infect host cells through the coordinated efforts of two envelope glycoproteins. The G glycoprotein attaches to cell receptors, triggering the fusion (F) glycoprotein to execute membrane fusion. G is a type II homotetrameric transmembrane protein responsible for binding to ephrinB2 or ephrinB3 (ephrinB2/B3) receptors. F is a homotrimeric type I transmembrane protein that is synthesized as a premature F0 precursor and cleaved by cathepsin L during endocytic recycling to yield the mature, disulfide-linked, F1 and F2 subunits. Upon binding to ephrinB2/B3, NiV G undergoes conformational changes leading to F triggering and insertion of the F hydrophobic fusion peptide into the target membrane. Subsequent refolding into the more stable post-fusion F conformation drives merger of the viral and host membranes to form a pore for genome delivery to the cell cytoplasm.

Recent Advances

 
Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
  • Background