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IDComponentsSizeRAB001-C01High-bind Plate1 plateRAB001-C02Mouse FMC63 scFv25 μgRAB001-C03FMC63 ADA Standard15 μgRAB001-C04Biotin-Mouse FMC63 scFv20 μgRAB001-C05Streptavidin-HRP50 μLRAB001-C06Coating Buffer12 mLRAB001-C0710xWashing Buffer50 mLRAB001-C08Blocking Buffer50 mLRAB001-C09Substrate Solution12 mLRAB001-C10Stop Solution7 mLProduct Overview
Anti-CD19 (FMC63) CAR Immunogenicity ELISA Kit is based on the bridging-ELISA method and is used to detect and quantitatively determine Anti-CD19 (FMC63) antibodies in CAR Immunogenicity assay test. The kit is designed to provide a reliable solution for detection and monitoring the level of ADA is important to fully understand the potential immune responses.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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Typical Data
Please refer to DS document for the assay protocol.
Detection of FMC63 ADA titer by bridging-ELISA Assay.
Immobilized Mouse FMC63 scFv can bind FMC63 ADA in 1:20 human serum, and then add Biotin-Mouse FMC63 scFv. Detection was performed using HRP-conjugated streptavidin with sensitivity of 19 ng/mL (QC tested).
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