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Product Details
Synonym
Integrin alpha 6 beta 1, ITGA6&ITGB1
Source
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1) (Accession # P23229-2 (ITGA6) & P05556-1 (ITGB1)).
Predicted N-terminus: Phe 24 (ITGA6) & Gln 21 (ITGB1)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB1, has a calculated MW of 116.8 kDa (ITGA6) and 83.7 kDa (ITGB1). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 kDa (ITGA6) and 100-118 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) at 1 μg/mL (100 μL/well) can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with a linear range of 0.01-0.156 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) captured on CM5 chip via anti-His antibody can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) with an affinity constant of 8.01 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) on Protein A Biosensor, can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 6.65 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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