Product Details
Synonym
Hemagglutinin/HA (Influenza Virus)
Source
Influenza A virus (Missouri/11/2025) HA (H1N1) Protein, His Tag (HA1-V52Ha) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - Ile 530 (Accession # EPI_ISL_20077100, GISAID).
Predicted N-terminus: Asp 18
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 63.0 kDa . The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 0.1 M Sodium citrate, 500 mM NaCl, 10% Glycerol, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
SDS-PAGE

Influenza A virus (Missouri/11/2025) HA (H1N1) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Influenza A virus (Missouri/11/2025) HA (H1N1) Protein, His Tag (Cat. No. HA1-V52Ha) is more than 90% and the molecular weight of this protein is around 240-280 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Influenza A virus (Missouri/11/2025) HA (H1N1) Protein, His Tag (Cat. No. HA1-V52Ha) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) (Cat. No. HA1-M757) with a linear range of 0.03-1 ng/mL (QC tested).
Protocol
Immobilized Influenza A virus (Missouri/11/2025) HA (H1N1) Protein, His Tag (Cat. No. HA1-V52Ha) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Human IgG1 (6D1) (Cat. No. HA1-M648) with a linear range of 0.03-1 ng/mL (Routinely tested).
Protocol
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