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Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) is a neutralizing mouse monoclonal antibody recombinantly expressed from human 293 cells, which provides higher batch consistency and long term security of supply.
We carry another premium grade FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (FM3-FY45G0), produced with the same production process but under more rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. It is designed for cell isolation and cell culture applications in the early preclinical stage.
Application
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).
Clone
Y45
Species
Mouse
Isotype
Mouse IgG1 | Mouse Kappa
Specificity
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
Immunogen
Recombinant FMC63 scFv derived from HEK293 cells
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Isotype Control
The isotype control is sold separately; please refer to Cat. No. DNP-FM1A1 for product information.
Recommended Dilution
1:50
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.5% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
Bioactivity-FACS
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-FY45P1) and Isotype control antibody respectively. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-FY45P1) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with PE-labeled anti-CD3 antibody and FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. Both PE and FITC positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
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