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分子别名（Synonym）
GLP-1-R, GLP-1R, glucagon like peptide 1 receptor
表达区间及表达系统（Source）
Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (GLR-H82D3) is expressed from human 293 cells (HEK293). It contains AA Arg 24 - Ser 432 (Accession # P43220-1).
Predicted N-terminus: Asp
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™).
The protein has a calculated MW of 67.9 kDa.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
- *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
产品展示
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Glutazumab with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Dulaglutide with a linear range of 0.5-31 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Dulaglutide captured on Protein A Chip can bind Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) with an affinity constant of 1.91 µM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
Glutazumab captured on Protein A Chip can bind Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) with an affinity constant of 30.6 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
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