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产品信息
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产品概述（Product Details）
ClinMax™ Human GM-CSF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human GM-CSF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M19 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
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应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human GM-CSF in serum, plasma and cell culture supernatants.
It is for research use only. -
流程图（Workflow）
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关键信息（Key Features）
AnalyteGM-CSFAssay TypeSandwich-ELISAReactivityHumanSensitivity10 pg/mLRange12.5 pg/mL-1600 pg/mLAssay Time1.75 hrSample TypeCell Culture Supernatants, Plasma, Serum.Sample volume50 uLFormat96-wells plate breakable into 12 x 8 wells strips -
存储（Storage）
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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质量管理控制体系（QMS）
产品数据图
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典型数据-Typical Data Please refer to Ds document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of GM-CSF is less than 10.0 pg/mL.
验证（Validation）
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批内差异（Intra-Assay Statistics）
Ten replicates of each of 3 samples containing different GM-CSF concentrations were tested in one assay. Acceptable criteria: CV＜10%.
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批间差异（Inter-Assay Statistics）
Three samples containing different concentrations of GM-CSF were tested in independent assays. Acceptable criteria: CV<15%.
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回收率（Recovery）
Recombinant GM-CSF was spiked into 5 human serum samples, and then analyzed. The average recovery of GM-CSF for serum samples is 102.82%.
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组分（Materials Provided）
IDComponentsSizeCRS017-C01Pre-coated Anti-GM-CSF Antibody Microplate1 plateCRB007-C02Human GM-CSF Standard32 μg×2CRB007-C03Biotin-Anti-GM-CSF Antibody Con. Solution100 μLCRB007-C04Biotin-Antibody Dilution Buffer8 mLCRB007-C05Streptavidin-HRP Con. Solution500μLCRB007-C06Streptavidin-HRP Dilution Buffer15 mLCRB007-C0720× Washing Buffer50 mLCRB007-C08Sample Dilution Buffer15 mL×2CRB007-C09Substrate Solution12 mLCRB007-C10Stop Solution6 mL
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