收藏产品 产品信息
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was engineered to not only express the NF-κB signaling response element, but also express the receptor human DR3 (TL1A receptor) (Gene ID: 8718). When stimulated with human TL1A protein, the TL1A/DR3 interaction drives NF-κB-mediated luminescence. Inhibition of TL1A binding to DR3 by either anti-TL1A or anti-DR3 antibodies results in a decrease in luminescence.
应用说明（Application）
• Screen for neutralizing antibodies blocking the stimulation of human TL1A protein.
生长特性（Growth Properties）
Suspension
筛选标记（Selection Marker）
Hygromycin B (20 μg/mL)
培养基（Complete Growth Medium）
RPMI-1640 + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品数据图
Application
Inhibition of human TL1A protein-induced reporter activity.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human TL1A protein (Cat. No. TLA-H5243) with a final concentration of 0.02 μg/mL. The EC50 of anti-human TL1A neutralizing antibody is approximately 0.023 μg/mL.
Protocol
Signaling Bioassay
Response to human TL1A protein (RLU).
This reporter cell was incubated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). The EC50 was approximately 0.013 μg/mL.
Protocol
Response to human TL1A protein (FOLD).
This reporter cell was incubated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). The max induction fold was approximately 19.54.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). Human TL1A protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 7-20.
Protocol
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背景
Tumor necrosis factor receptor superfamily member 25 (TNFRSF25) is also known as Apo-3, Death receptor 3 (DDR3 or DR3), Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor TRAMP, Lymphocyte-associated receptor of death, Apo-3, which is a member of the TNF-receptor superfamily. TNFRSF25 is a homodimer protein, which can Interact strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. TNFRSF25 is receptor for TNFSF12 / APO3L / TWEAK.
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