积分兑换
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- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The CHO/Human CD79A&CD79B Stable Cell Line was engineered to express the receptor full length human CD79A (Gene ID: 973) and CD79B (Gene ID: 974). Surface expression of human CD79A and CD79B was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based CD79A or CD79B binding assay.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Complete Growth Medium）
F-12K + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品数据图
Receptor Assay
Expression analysis of human CD79A on CHO/Human CD79A&CD79B Stable Cell Line by FACS.
Intracellular staining was performed on CHO/Human CD79A&CD79B Stable Cell Line or negative control cell using FITC-labeled anti-human CD79A antibody after fixation and permeabilization.
Protocol
Expression analysis of human CD79B on CHO/Human CD79A&CD79B Stable Cell Line by FACS.
Cell surface staining was performed on CHO/Human CD79A&CD79B Stable Cell Line or negative control cell using APC-labeled anti-human CD79B antibody.
Protocol
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背景
CD79a and CD79b heterodimers are the important signaling components of B cell receptor (BCR) complex which plays a crucial role in B cell development and antibody production. BCR complexes are composed of a ligand-binding receptor (membrane immunoglobulin; mIg), non-covalently associated with the signaling com-ponent, a disulfide-linked heterodimer of CD79a (Igα) and CD79b (Igβ). Both CD79 subunits consist of an Ig-like domain, a transmembrane (TM) region, and a longer cytoplasmic tail (CYT) containing an immune-receptor tyrosine-based activation motif (ITAM).
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