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背景（Background）
The Chemiluminescent Substrate Solution (HRP Marker) is a specific triggering reagent for peroxidase labels such as horseradish peroxidase (HRP) and can be used in ELISA procedures. This substrate is an enhanced chemiluminescent substrate enables detection at femtogram range of target by oxidizing luminol in the presence of horseradish peroxidase (HRP) and peroxide. This product is provided in two liquid buffer of Solution A (Luminol-Based Solution) and Solution B (Peroxide Solution). When used, mix Solution A and Solution B in equal volume by 1:1, and it gives a rapid light emission.
应用说明（Application）
The chemiluminescent substrate solution is used to trigger HRP labels to give a quite rapid light emission in chemiluminescence technology.
存储（Storage）
Stable for 1 year at 4°C. Do not freeze. Product is shipped at ambient temperature.
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活性（Bioactivity）-ELISA
When used the Chemiluminescent Substrate Solution (HRP Marker) (Cat. No. ABK-002) in a sandwich CLEIA Assay for detection of Human IFN-gamma, immobilized Monoclonal Anti-IFNγ antibody, Human IgG1 at 2 μg/mL, add increasing concentrations of Human IFN-gamma, premium grade (Cat. No. IFG-H4211) and then add Biotinylated Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG at 0.05 μg/mL. Detection was performed using Streptavidin-HRP, Horseradish peroxidase conjugated Streptavidin (Cat. No. STN-NH913) with sensitivity of 7.8 pg/mL (QC tested).
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