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抗体来源（Source）
FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 (RIB-FY35c) are expressed from human 293 cells (HEK293).
应用（Application）
ELISA
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1/kappa
特异性（Specificity）
Recognizes Rituximab specifically, no cross reactivity with other humanized antibodies.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
产品数据图
活性（Bioactivity）-ELISA
Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind FITC-Labeled Anti-Rituximab Antibody, Mouse IgG1 (Cat. No. RIB-FY35c) with a linear range of 0.01-0.3 μg/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Anti-CD20 (Rituximab) CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Anti-Rituximab Antibody, Mouse IgG1 (Cat. No. RIB-FY35c) and isotype control antibody respectively. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
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背景
Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids
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